Concrete Microbial self-healing overview

Concrete is a most extensively used material in construction; however, cracks in concrete are
unavoidable. There is a new technology that can heal the cracks by precipitated calcium
carbonate called as microbial self-healing concrete which reduces the coefficient of
permeability. The self-healing/self-repairing concrete with the addition of fibers can be used in
construction industries to enhance the strength and durability of concrete. Fibers may reduce
the crack width by bridging action and bacteria develop a filling material in that bridge portion.

This improves the durability and strength of bacterial concrete. In the present study, four
different mixes Self‐repairing of concrete cracks by using bacteria and fiber:
are prepared, namely normal concrete, bacterial concrete, fiber-reinforced concrete, and
bacterial concrete, with the addition of fibers. The healing/repairing efficiency of concrete is
measured in terms of electrical resistivity and compressive strength of concrete on pre-cracked
samples and healed samples. Further, the results are correlated with the scanning electron
microscope and energy-dispersive X-ray spectrometer analysis. The results and analysis that
carried out show substantial enhancement in the durability and strength of concrete with the
addition of fibers in bacterial concrete.

1 Introduction:
In construction industry, concrete is the most extensively utilized material in the world and is
the second most consumable material after water. Concrete is strong in compression and
week in tension and has limited ductility, and cracks in concrete are inevitable [1]. There are
so many repairing techniques accessible to repair the concrete structures. The development
of cracks reduces the life span of concrete. The repairing costs of concrete structures are
increasing day by day. A study estimates that the expense of concrete production is $65 to
$80/m3, while that of repairing cracks and maintenance is approximately $147/m3 [2].
However, these methods are not eco-friendly and not economic [3, 4].
It should be noted that, in case of reinforced concrete structures, cracks are generally not
considered to be a failure or damage and cracking does not specify a safety problem. The concrete loses its ability to protect the reinforced steel against corrosion in case of wider crack lengths. One of the major reasons for permanent concrete structure failure is the corrosion of steel. Micro cracks do not affect the strength of concrete at the initial time. However, micro-cracks result in the formation of a network of cracks and contribute largely to the concrete permeability. This results in a reduction of resistance of the concrete against the ingress of aggressive substances [5, 6]. In certain places like water sewage pipes and nuclear power plants, repairing of cracks manually is a tedious task and also hazardous [7]. In this regard, the self-repairing mechanism is utmost essential for this purpose. Further, the practical experiences and experimental investigations on cement materials demonstrated that cracks can be healed by themselves, termed as autogenously healing. The autogenously healing is observed in cracks of maximum width between 0.1 to 0.2 mm. In young concrete, it was reported that 0.1-mm-wide cracks take several days for healing, whereas several weeks are required for 0.2-mm- wide cracks. The autogenously healing in younger concrete is possible due to more unhydrated cement. However, it is difficult for autogenously healing at later ages of concrete [8–10]. The research on preventing interventions to limit the formation of cracks has been increased in the recent past so as to achieve higher structural performance [11]; further,
the monumental response to the degradation of concrete at the micro-level by adding the self healing materials has also drawn the attention of researchers. For this purpose, different healing agents such as polymers and biotechnological healing agents are used in order to improve the durability and strength of concrete [12, 13]. The increase in the focus of researchers in this aspect is depicted in Fig. 1 based on the publications in recent years [1, 8]. The concept of self-healing is a well-known ancient phenomenon [14, 15]. However, its capacity for the purpose of crack-healing is limited in most of the concrete structures [16, 17]. In recent years, bacteria-precipitated CaCO3 has been an alternative and eco-friendly technique for self-healing of cracks [18, 19]. This technique has been proposed by Jonkers et
al. for self-healing without human interface [20, 21]. In [22] observed that using bacteria in
concrete healed most of the cracks (up to 0.3mm) in 5 days and fill the surface totally in 20
days. The contrast test applied to concrete in [23] reported the microbial concrete repair cracks
of wider length up to 470μm while in control concrete 210 μm only. Microbial concrete heals
the cracks with the precipitated calcium carbonate. How- ever, the properties of concrete after
healing are not much improved. Also, limited literature reports regarding the performance of concrete after healing of cracks. In this regard, an endeavor has been made to examine the performance of concrete after healing of cracks. It is Fig.1 Year-wise number of publications for phrase search “concrete healing” [1, 8] well known that the addition of fiber reduces the crack width and also the number of cracks in concrete by virtue of bridging action [24, 25]. Hence, in this study fibers have been used due to its eco-friendliness and high tensile strength. It also has the advantage of minimizing the crack widths and number of cracks by its bridging action and high tensile strength. The main objective of this paper is to study the self-repairing capacity of bacterial concrete with fiber in it and investigations on the enhancement of durability and strength of concrete.

*Kunamineni Vijay, [email protected] | 1Department of Civil Engineering,
National Institute of Technology, Raipur 492010, India.
Received: 9 August 2022 / Accepted: 1 October 2022 / Published for JC Family Concrete)

2 Materials and methods:
2.1 Materials
In this study, ordinary Portland cement (OPC), fine aggregate, coarse aggregate, b-s bacteria, fiber, and calcium lactate are used for making the concrete. The OPC has been utilized in this study with 3.1 specific gravity and cement tested as per Indian Standards [26,27]. Coarse aggregate of size less than 20 mm (20 mm nominal size) and Zone II fine aggregate were used with a specific gravity of 2.79 and 2.7, respectively, confirming to Indian Standards [28, 29]. Normal port- able water available in the laboratory has been used for the preparation of concrete. B-S bacteria are procured from Retron Pro Biotech Ltd., Vizag, and are shown in Fig. 2. The test details of bacteria are given in Table 1. From this, it is confirmed that the procured spores of bacillus subtilis are gram-positive bacteria. Based on the literature [11, 17], 105 cfu/ml is the
B-S spore powder.

Research:
1: Test data for identification of bacteria
Test performed Starch test
Gram-staining reaction
Indicator used
Starch and Iodine Solution
Crystal violet
Elastic modulus (GPa) Tensile strength (MPa) Length (mm)
Specific gravity
Color observation
A dark color of the whole medium except colony and its surrounding
Purple
Properties of basalt fiber Parameter
Result Positive
Gram positive
Value
93 3200–3850 12
2.7

3: Calcium lactate powder
Table 2 Physical properties of calcium lactate Parameter
Appearance Solubility Arsenic Heavy metals Iron
Loss on drying
Value
White powder Soluble in water
≤ 2 parts per million ≤ 20 parts per million ≤ 80 parts per million 23.50%

4: Optimum concentration for improving the strength of concrete same concentration of bacteria which is used in this study. Calcium lactate is used as a nutrient source for bacteria; it was procured from Triveni Chemicals, Gujarat. The appance of calcium lactate. The test details of the calcium lactate are given in Table 2. As per literature, [17] 0.5% (w.r.t weight of cement) dos- age of calcium lactate was added to concrete for better strength properties. Fiber used in this study is procured from the Hydro Design Management Co. Pvt. Ltd, Noida, and details of properties are given.

2.2 Concrete mix design:
In this study, OPC, fine aggregate, coarse aggregate, b-s bacteria, fiber, and calcium lactate are used for making the concrete. Three water–cement ratios (0.45, 0.4, and 0.35) are utilized to prepare the concrete mix. The concrete mixes were prepared as per Indian Standards IS: 10262-2009 [30]. The major variable in the concrete mix was water–cement ratio, and bacteria, calcium lactate, and basalt fiber are the additional materials.

2.3 Workability of concrete:
The property of fresh concrete as workability was measured by slump cone test and compaction factor (CF) test as per IS: 1199-1956. The slump value indicates the stiffness of the fresh mix to work with. Another popular and commonly used test to know the workability of concrete is the CF test. The test results of the CF are more sensitive and reliable for low workable mixes.

2.4 Mechanical properties:
The harden properties of concrete are measured by compressive and flexural strength tests. The compressive strength of concrete was tested on 100 × 100 × 100 mm size cubes as per IS: 516-1959 [31]. The flexural strength of concrete was tested on beam samples of standard dimensions 500 × 100 × 100 mm as per IS: 516-1959. The compressive strength is tested at an age of 7, 28, and 56 days of water cured samples by utilizing compression testing machine. The flexural strength is tested at an age of 28- and 56-day water cured samples. For determination of compressive and flexural strengths, the average value of three samples is taken.

2.5 Electrical resistivity of concrete:
1222.33 161.54 1222.33 161.54
1222.33 1222.33
Nil 161.54 105 161.54 105
The electrical resistivity test on concrete was performed to know the quality of concrete in terms of voids and internal cracks. The test has been performed at an age of 7-, 28-, and 56- day cured samples, and for each test, the average value of three samples is used for the determination of electrical resistivity of concrete. For the measurement of electrical resistance of concrete Leader RCONTM has been used at a predetermined location. The test has been performed on three cubes for each mix in three different conditions: undamaged samples, damaged samples, and healed samples. The electrical resistivity (ρ in Ω∙m) of concrete for an average value of electrical resistance (R in Ω∙), length (l in m) and area (A in m2) is given by [17]: 𝜌 = RA (1) l

2.6 Microstructure analysis:
For analyzing the microstructure of concrete, SEM analysis has been carried out on ZEISS SEM machine. The 28-day cured samples are collected and immersed in acetone to stop the further hydration process. These collected samples are prepared with the size of 1-cm cubes with smooth surface. These samples have been coated with gold in sputter coater for obtaining high-resolution SEM images. Simultaneously, EDX analysis is performed for knowing the major elements present on the surface.

Slump Value (mm)
90 80 70 60 50 40 30 20 10
0
w/c=0.45 w/c=0.4 w/c=0.35
Mix-1 Mix-2 Mix-3 Mix Details
Mix-4
Compaction factor test results of different concrete mixes 2.7 X‐ray diffraction (XRD) analysis for the deposited material at the cracks. The deposited material at the crack is collected from the concrete samples to know the chemical composition using XRD analysis. The collected samples were ground and sieved from 75 microns. Compressive strength results of different concrete mix.

3 Results and discussion:
3.1 Workability of concrete
The combined effect of bacteria, calcium lactate, and basalt fiber on workability of concrete mix has been examined. The results show that the addition of calcium lactate increases the slump value, while, the fiber decreases the slump value of respective concrete. The addition of bacteria, calcium lactate, and fiber together gives slump value more than the basalt fiber reinforced mix and shows a good workability. It is noticed that similar results have been obtained for compaction factor tests also. Similar to normal concrete mix, the bacterial concrete also shows a proportional change in workability with water–cement ratio. The calcium lactate works as a retarding agent in concrete; it increases the setting time and fluidity of concrete [15]; due to this, the workability of bacterial concrete increases, whereas basalt fibers absorb certain moisture content during the mixing and also increase the friction between fibers and cement [32]. This activity decreases the workability of fiber-reinforced concrete and bacterial concrete embedded with basalt fiber. The addition of fiber, bacteria, and calcium lactate gives good workable concrete nearly same as normal concrete.

3.2 Mechanical properties of concrete:
The strength results of concrete are depicted. The compressive strength is significantly improved in bacterial concrete and bacterial concrete embedded with basalt fiber. Similar trend has been followed in flexural strength also. Confirms that microbial activity plays an important role in improving the compressive.
Compaction Factor
0.9 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1
0
w/c=0.45 w/c=0.4 w/c=0.3

Mix-1 Mix-2 Mix-3 Mix Details
Mix-4
Compressive Strength (MPa)
70 60 50 40
30 20 10
0
7 Days 28 Days 56 Days
Mix Details (For 0.45 w/c)
Mix Details (For 0.4 w/c)
Mix Details (For 0.35 w/c)

Flexural strength results of different concrete mixes
Flexural Strength (MPa)
9 8 7 6 5 4 3 2 1 0
28 Days 56 Days
Mix Details (For 0.45 w/c)
Mix Details (For 0.4 w/c)
Mix Details (0.35 w/c) strength. Iis noticed that there is 20%, 24%, and 27% enhancement in compressive strength of bacterial concrete as compared to control mix for a water–cement ratio of 0.45, 0.4, and
0.35. The maximum compressive strength was achieved using higher grade of concrete (with lower water–cement ratio) in the presence of bacillus subtilis microbes. The improvement in strength of bacterial concrete as compared to normal concrete is due to the formation of calcite in bacterial concrete; it fills the voids and improves the strength. This is confirmed by the microstructure analysis of concrete, which will be discussed in Sect. 3.4. It was also reported that CaCO3 generated by bacteria reduces porosity and enhances the compressive strength. Similar results are obtained in [11, 17], as the addition of bacteria enhances the concrete strength by filling the pores with CaCO3 produced by metabolic activity of bacteria. The additions of basalt fibers enhance the compressive and flexural strength of normal concrete as well as bacterial concrete. The addition of fiber improves the load-carrying capacity by bridging action in cracks. As the basalt fiber will carry further increasing loads after the first crack, which improves the overall strength of the concrete, the bacterial concrete enhances the strength of concrete and performs better workability. The relationship between workability and compressive strength of concrete , it is noticed that as the water–cement ratio increases the workability of the concrete increases and the strength decreases. Similar trend was observed for all concrete mixes. However, bacterial concrete shows improvement in workability and strength as compared to control mix. In fiber-reinforced concrete, the workability of concrete decreases whereas the strength of the concrete showed a considerable improvement. The addition of fibers in concrete increases the friction between fibers and cement; this activity decreases the workability of fiber-reinforced concrete. But in bacterial concrete, the addition of fibers shows the improvement in strength and the workability is nearly equal to normal concrete.
For knowing the recovery properties of concrete after healing, the concrete samples are damaged by applying 60% of load-carrying capacity at an age of 28 days. Further these cubes are kept in water curing for healing purpose. After 28 days compressive strength was determined for healed samples and compared with the undam- aged samples at an age of 56 days. Figure 10 shows the comparison of healed samples compressive strength that was subjected to damage by preloading and undamaged samples at an age of 56 days. Bacterial concrete shows considerably higher strengths after healing as compared to control mix. The strength recovery of bacterial concrete with the addition of basalt fiber shows better results than all the other concrete mixes. The strength difference in 56 days for undamaged samples and healed samples can be measured as the unrecovered strength. The unrecovered strength in Mix 4 is 16.7%, whereas in MIX 1 it is 30.75% for 0.4 water–cement ratio. The unrecovered strength in bacterial concrete and bacterial concrete with addition of fibers is very little. It confirms that the recovery rate is very high in bacterial concrete and bacterial concrete with basalt fibers as compared to other mixes. Bacterial concrete and bacterial concrete with the addition of basalt fibers show good results in recovery of concrete properties after preloading.

3.3 Electrical resistivity of concrete
The electrical resistivity test on concrete was performed to know the quality of concrete in terms of voids and internal cracks. The test has been performed at an age of 7-, 28-, and 56-day cured samples. The results obtained are listed in Fig. 11. The results validate the significant improvement in electrical resistivity of bacterial concrete embedded with fiber.
Compressive Strength (MPa)
Compressive Strength (MPa)
50 60
45
40
50
35
40
30
25 30
Compressive Strength (MPa) 20
15
20
10
10
5
00
60 65 70 75 80 30 35 40 45 50 55
Slump Value (mm)
(b)
70 60 50 40 30 20 10
Compressive Strength (MPa)
70 60 50 40 30 20 10
Slump Value (mm)
(a)
0.35 w/c
0.4w/c
0.45 w/c
0.35 w/c
0.4 w/c
0.45 w/c
0
60 65 70 75 80 85
Slump Value (mm)
(c)
0
50 55 60 65 70
Slump Value (mm)
(d)
0.35 w/c
0.4w/c
0.45 w/c
0.35 w/c
0.4w/c
0.45 w/c
Fig. 9 Relationship between workability and compressive strength of concrete for: a MIX 1, b,
MIX 2 c MIX 3, d MIX 4
Fig. 10 Comparison of compressive strength of healed samples damaged by preloading and
undamaged samples at an age of 56 days
Compressivestrength (MPa)
70 60 50 40 30 20 10
0
Preloaded
MIX-1 MIX-2 MIX-3 MIX-4 Mix Details (For 0.45 w/c)
Undamaged
MIX-1 MIX-2 MIX-3 MIX-4 Mix Details (For 0.4 w/c)
MIX-1 MIX-2 MIX-3 MIX-4 Mix Details (For 0.35 w/c)
limited ductility and exhibits brittle failure. The presences of internal micro-cracks in concrete are the root cause of durability issues in concrete. Controlling them is essential to ensure and enhance the performance of concrete composites. The addition of bacteria and basalt fiber in concrete shows improvement in electrical resistivity. This validates the advantage of using bacteria and basalt fiber in concrete. The cubes are pre-cracked by applying the compression load at an age of 28 days, and these
cubes are kept water curing for healing purpose. The electrical resistivity test has been performed on preloaded (pre-cracked) cubes, and the same test has been performed on healed concrete samples for knowing the rate of self-repairing of concrete. Figure 12 depicts the results of pre-cracked and healed samples at an age of 28 days and 56 days.
Electrical resistivity of different concrete mixes
Electrical Resistivity (kΏ-cm)
35 30 25 20 15 10
5 0
7 Days 28 Days 56 Days
MIX-1 MIX-2 MIX-3 MIX-4
Mix Details (For 0.45 w/c)
MIX-1 MIX-2 MIX-3 MIX-4
Mix Details (For 0.4 w/c)
MIX-1 MIX-2 MIX-3 MIX-4
Mix Details (For 0.35 w/c)
Fig. 12 Electrical resistivity results of pre-cracked and healed samples
Electrical Resistivity (kΏcm)
35 30 25 20 15 10
5 0
Pre Loaded (28 days)
28 days
Healed Sample (28+28 days) 56Days
MIX-1 MIX-2 MIX-3 MIX-4 Mix Details (For 0.45 w/c)
MIX-1 MIX-2 MIX-3 MIX-4 Mix Details (For 0.4 w/c)
MIX-1 MIX-2 MIX-3 MIX-4 Mix Details (For 0.35 w/c)
The self-repairing capacity of concrete has been greatly enhanced with bacteria and basalt fiber. The healing percentage is high in concrete with lower water–cement ratio as compared to higher water–cement ratios in the presence of bacteria and basalt fiber. This is due to the production of more CaCO3 and the basalt fiber, which acts as a bridging material between cracks.

3.4 Micro structure analysis
The SEM and EDX analyses of normal and bacterial concrete are illustrated in Fig. 13. It is noticed that the presence of tetrahedron and pyramid shapes in bacterial concrete shows the presence of calcite. It means the pores in concrete mix are filled with deposited calcite, resulting in enhancement of durability and strength of concrete [21, 22]. From EDX analysis, it is noticed that the amount of Ca is obtained for bacterial concrete which is higher than control concrete. The elemental analysis confirms the pre ence of higher amount of Ca in bacterial concrete. SEM and EDX analyses confirm the presence of CaCO3 in bacterial concrete. This fills pores and tiny cavities in concrete and improves the durability and strength of concrete.

3.5 X‐ray diffraction (XRD) analysis for the deposited material at the cracks XRD analysis was done for the deposited material at the cracks after healing the cracks. Figure 14 shows that the XRD analysis of deposited material. From Fig. 14 it is noticed that the crystalline peaks are present at an angle (2Ɵ) of 29.6°, 36.2°, 39° and 47.7°. These peaks represent the presence of calcite in the material. The formation of calcite by bacteria is the reason for the improved durability and strength of concrete after healing the cracks.

4 Conclusions
The investigation of using bacteria along with fiber in concrete for improving and recovery of concrete properties of damaged samples has been explored in this paper. Workability, mechanical properties, and recovery of strength for samples healed by CaCO3 precipitation by microbes have been investigated. The addition of fiber in bacterial concrete enhances the compressive strength as well as flexural strength. The workability FSEM analysis of a control concrete and b bacterial concrete, and EDX analysis of c control
concrete and d bacterial concrete XRD analysis of deposited material at cracks after healing slightly decreases with the addition of fiber; how- ever, bacterial concrete shows good workability even with the addition of fibers. The addition of fiber contributes to the recovery of properties of healed samples. SEM analysis of concrete confirms the presence of calcite in bacterial concrete. The calcite fills the voids and micro-cracks in concrete and enhances the concrete performance. The compressive strength has been observed to be recovered using fiber in bacterial concrete. The electrical resistivity test results also conforms the recovery of concrete properties after healing the cracks. Further XRD analysis also confirms that the deposited material in the cracks is calcite; this is the reason for improving the recovery properties of concrete * The life span of concrete sharply decreases with age, the formation of cracks on its surface ultimately leads to corrosion
* This formula addresses the problem through a process known as
Microbially Induced Calcite Precipitation (MICP)
* University research has shown that MICP increases compressive strength,
split tensile strength and flexural strength
* Formula contains Bacillus subtilis a non-pathogenic, naturally occurring soil bacterium
* Bacillus subtilis will produce secondary metabolites that promotes the
formation of calcite deposits inside concrete pores
* This results in increased density and strength of the concrete
* Bacillus subtilis can be added directly to concrete mix or applied to existing foundation walls
CONTAINS NON-PLANT FOOD INGREDIENTS
Contains Microbial Nutrients and Microbials
APPLICATION SPECIFICS FOR ADDING TO CONCRETE MIX
* Target ratio is 1 x 106 colony forming units per ml of water in the mix
* The formula contains 5 x 109 cfu per gram therefore you require 1 gram of material per
5,000 ml / 1.32 gal of water utilized in the mix
* Add product to cement at time of preparation @ prescribed ratio of 1 gram per 1.32 gal of
water utilized in mix * Do not tank mix more than you will utilize within 24 hours
APPLICATION SPECIFICS FOR APPLYING TO EXISTING CONCRETE.
* Utilize 23 grams / 0.05 lbs of product for every 40 square feet of exiting foundation
* Thoroughly dilute product in a sufficient volume of potable water to achieve desired coverage
* Apply homogeneously to foundation walls inside and outside once ambient air temperature is
50 degrees F * Utilize a fine mist setting to facilitate absorption, avoid runoff
* Do not tank mix more than you will utilize within 24 hours
* Keep container sealed, when stored properly product has 24-month shelf life
MANUFACTURED BY JC FAMILY AG